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-   -   Determination of flavonoids in hawthorne (http://chemistryhelpforum.com/showthread.php?t=146495)

New Dec 15th 2017 01:34 PM

Determination of flavonoids in hawthorne
 
Hello, I have 2 questions from an experiment's procedure:

First, When preparing a crude extract from Hawthorne for determining its flavonoids, why do we add 1cc methenamine 0.5% to the solvent (20cc acetone and 2cc hcl 25%) before reflux?

Second, what happens when we add AlCl3 to the extract before spectrophotometry? I need the mechanism for the complexation reaction

Here is the method: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541481/


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