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Old Mar 21st 2017, 12:30 AM   #1
tashian
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ABTS-TEAC calculation

Hi,

I'm a grad student and would appreciate your help with some calculations:

I was using the TEAC protocol for antioxidants with ABTS. The numbers are as follows:
Sample reading 0.35
Trolox reading 0.4
Blank reading 0.534
Trolox concentration 0.25 mM
sample weight 0.3 gr
final extraction volume 0.2 ml

My calculation was:
TE=((sample reading-blank)/(trolox reading-blank))*trolox concentration
TEAC=TE*(final volume/sample weight)/sample weight.
To my understanding, the units in such case would be mM TE/gr.


Can you please help me with calculating the TEAC value and understanding its units? I have seen all kinds of units for this (mmol, uM) and wonder why is it so.

Many thanks!
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