"Apply 200 ul of 1 nM Fluorescein(375g/mol) in 0.01 M NaOH solution to wells."

Assuming total volume is 1L - 10mL NaOH with 990mL H20 should give .01M NaOH

I can't figure out the 2nd part. Thinking of it logically, 1M fluorescein would be achieved by adding 375g of it, so I assume .000000375g / L would be 1nM.

This doesnt seem to make any sense since it's not practical to weight out that small amount so it can't be what the instructions are asking.

Any help on this is greatly appreciated. Thanks!