Help on homework

May 2017
(8) An artificial myoglobin our lab recently designed has two independent O2 binding sites. The experimentally observed Kd for the first site was 600 nM.

(a) Estimate the observed Kd for the second.

(b) The ΔGfolding for the protein in the absence of O2 is -5.5 kcal/mol. Assuming the unfolded protein cannot bind O2, what is the ΔGfolding in O2 –saturated solution (2 mM)?